6163银河官网

6163银河官网-石贵阳-正高--

国家级重点学科

一级学科博士学位授予点

“双一流”建设学科

ENGLISH

师资队伍
Faculty and staff

陈晟

陈 晟

职称:副教授、硕士生导师

学历/学位:研究生、博士

电话:0510-85326653

电子邮箱:chensheng@jiangnan.edu.cn

简介

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长期从事食品生物技术、酶工程和发酵工程方面的研究,主要承担《蛋白质工程》、《分子生物学实验》、《高级生物化学》等课程的讲授工作。近年来主持国家省部级项目6项。获国家技术发明二等奖、教育部一等奖及中国商业联合会特等奖各一项,参与编写专著及教材各一本。在国内外主流学术期刊上发表学术论文60余篇,其中SCI论文34篇,包括Biotechnology Advances、J Biol Chem、Appl Environ Microb、J Agr Food Chem、Microb Cell Fact等国际权威杂志,最高影响因子12.831。申请专利36项,其中授权专利27项,参与完成科技成果鉴定5项。

学习工作经历

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1999-2003 江南大学,生物工程专业,学士

2003-2006江南大学,发酵工程专业,硕士

2006-2009江南大学,发酵工程专业,博士

2009-至今 江南大学,食品科学与技术国家重点实验室,副教授

2012-2013 哈佛大学,化学与化学生物学院,访问学者

主要成果

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一、论文(论著)发表情况

1     Sheng Chen, Yufei Wu, Lingqia Su, Jing Wu*. Contribution of disulfide bond to the stability of Thermobifida fusca cutinase. Food Bioscience, 2019, 27, 6–10.

2     Lei Wang, Sheng Chen, Jing Wu*, Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli, BMC Biotechnology, 2018, 18: 72

3     Fei Wang, Jing Wu, Sheng Chen*. Preparation of gentiooligosaccharides using Trichoderma viride β-glucosidase. Food Chemistry, 2018, 248: 340-345

4     Ruoyu Hong, Lingqia Su, Sheng Chen, Zhu Long, Jing Wu*, Comparison of cutinases in enzymic deinking of old newsprint, Cellulose, 2017, 24: 5089-5099

5     Lina Liu, Sheng Chen, Jing Wu*, Phosphoenolpyruvate: glucose phosphotransferase system modification increases the conversion rate during L-tryptophan production in Escherichia coli, Journal of Industrial Microbiology & Biotechnology, 2017, 44 : 1385-1395

6     Zhu Li, Xuguo Duan, Sheng Chen, Jing Wu*, Improving the reversibility of thermal denaturation and catalytic efficiency of Bacillus licheniformis α-amylase through stabilizing a long loop in domain B, Plos One, 2017, 12(3): e0173187

7     Sheng Chen, Yanjun Xiong, Lingqia Su, Lei Wang, Jing Wu*, Position 228 in Paenibacillus macerans cyclodextrin glycosyltransferase is critical for 2-O-D-glucopyranosyl-L-ascorbic acid synthesis, Journal of biotechnology, 2017, 247(10): 18-24

8     Sheng Chen, Huihui Gao, Jian Chen, Jing Wu*, Surface modification of polyacrylonitrile fibre by nitrile hydratase from corynebacterium nitrilophilus, Applied Biochemistry and Biotechnology, 2014, 174(6): 2058-2066

9      Zhaofeng Li, Sheng Chen, Zhengbiao Gu, Jian Chen, Jing Wu*, Alpha- cyclodextrin: enzymatic production and food applications, Trends in Food Science and Technology, 2014, 35(2): 151-160

10  Hui Deng, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*, Heterologous expression and biochemical characterization of glucose isomerase from Thermobifida fusca, Bioprocess and Biosystems Engineering, 2014, 37(6): 1211-1219.

11  Yingchun Guo, Sheng Chen, Lingqia Su, Jing Wu*, Jian Chen, Cloning, expression and characterization of polyamidase from Nocardia farcinica and its application on polyamide modification, Biotechnology and Bioprocess Engineering, 2013, 18(6): 1067-1075

12  Sheng Chen, Lingqia Su, Jing Wu*, Jian Chen, Cutinase: characteristics, preparation, and application, Biotechnology Advances, 2013, 31(8): 1754–1767

13  Fan Zhang, Xuguo Duan, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*, The addition of Co2+ enhances the catalytic efficiency and thermostability of recombinant glucose isomerase from Thermobifida fusca, Process Biochemistry, 2013, 48(10): 1502-1508

14  Yufei Wu, Shuguang Yuan, Sheng Chen, Dan Wu, Jian Chen, Jing Wu*, Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus β-galactosidase, Food Chemistry, 2013, 138(2-3): 1588- 1595 

15  Berhane T. Tesfai, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*, Effect of organic solvents on the yield and specificity of cyclodextrins by recombinant cyclodextrin glucanotransferase (CGTase) from Anaerobranca gottschalkii, Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2013, 77: 147-153

16  Xuguo Duan, Sheng Chen, Jian Chen, Jing Wu*, Enhancing the cyclodextrin production by synchronous utilization of isoamylase and α-CGTase, Applied Microbiology and Biotechnology, 2013, 97(8): 3467-3474

17  Bin Li, Lei Wang, Lingqia Su, Sheng Chen, Zhaofeng Li, Jian Chen, Jing Wu*, Glycine and triton X-100 enhanced secretion of recombinant α-CGTase mediated by OmpA signal peptide in Escherichia coli, Biotechnology and Bioprocess Engineering, 2012, 17(6):1128-1134

18  Berhane T. Tesfai, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*, Strategies for enhancing extracellular secretion of recombinant cyclodextrin glucanotransferase in E. coli, Applied Biochemistry and Biotechnology, 2012, 167(4):897-908

19  Dan Wu, Sheng Chen, Ning Wang, Jian Chen, Jing Wu*, Gamma-cyclodextrin production using cyclodextrin glycosyltransferase from Bacillus clarkii 7364, Applied Biochemistry and Biotechnology. 2012, 167(7):1954-1962

20  Lingqia Su, Sheng Chen, Li Yi, Ronald W Woodard, Jian Chen, Jing Wu*, Extracellular overexpression of Recombinant Thermobifida fusca cutinase by alpha-hemolysin Secretion System in E. coli BL21(DE3),  Microbial Cell Factories, 2012, 11(1):8

21  Zhijun Zhao, Sheng Chen, Dan Wu, Jing Wu*, Jian Chen. Effect of gene knockouts of L-tryptophan uptake system on the production of L-tryptophan in Escherichia coli, Process Biochemistry, 2012, 47(2):340-344

22  Carla Silva, Shi Da, Nádia Silva, Teresa Matamá, Rita Araújo, Madalena Martins, Sheng Chen, Jian Chen, Jing Wu, Casal M, Artur Cavaco-Paulo*, Engineered Thermobifida fusca cutinase with increased activity on polyester substrates, Biotechnology Journal. 2011, 6(10):1230-1239

23  Zhiguo Liu, Li Zhu, Kongliang Zhu, Sheng Chen, Jian Chen*, Jing Wu*, Effect of silent mutations in translational initial region on the production of recombinant cutinase in Escherichia coli, Current Microbiology, 2011, 62(4):1302-1307

24  Sheng Chen, Bin Li, Ruoyu Hong, Jian Chen*, Jing Wu*, The number of signal peptide cleavage site is critical for extracellular production of recombinant Thermobifida fusca cutinase, Process Biochemistry, 2011, 46:1867-1870

25  Yao Zhang, Sheng Chen, Miao He, Jing Wu*, Jian Chen, Qiang Wang, Effects of Thermobifida fusca cutinase-carbohydrate-binding module fusion proteins on cotton bioscouring, Biotechnology and Bioprocess Engineering, 2011, 16(4): 645-653

26  Sheng Chen, Zhiguo Liu, Jian Chen*, Jing Wu*, Study on improvement of extracellular production of recombinant Thermobifida fusca cutinase by Escherichia coli, Applied Biochemistry and Biotechnology, 2011, 165(2):666-675

27  Zhijun Zhao, Chun Zou, Yixing Zhu, Jun Dai, Sheng Chen, Dan Wu, Jing Wu*, Jian Chen*, Development of L-tryptophan production strains by defined genetic modification in Escherichia coli, Journal of Industrial Microbiology and Biotechnology, 2011, 38:1921-1929.

28  Jing Cheng, Dan Wu, Sheng Chen, Jian Chen, Jing Wu*, High-Level Extracellular production of α-cyclodextrin glycosyltransferase with recombinant Escherichia coli BL21(DE3), Journal of Agricultural and Food Chemistry,  2011, 59:3797–3802

29  Yao Zhang, Sheng Chen, Meng Xu, Artur Cavoco-Paulo, Jing Wu*, Jian Chen*,  Characterization of Thermobifida fusca cutinase–carbohydrate-binding module fusion proteins and their potential application in bioscouring, Applied and Environmental Microbiology, 2010, 76(20):6870-6876

30  Dongli Chen, Xing Tong, Shangwei Chen, Sheng Chen, Dan Wu, Shuguang Fang, Jing Wu*, Jian Chen*. Heterologous expression and biochemical characterization of alpha-glucosidase from Aspergillus niger by Pichia pastroris, Journal of Agricultural and Food Chemistry, 2010, 58(8):4819-4824

31  Runrong Ding, Zhaofeng Li, Sheng Chen, Dan Wu, Jing Wu*, Jian Chen*, Enhanced secretion of recombinant α-cyclodextrin glucosyltransferase from E. coli by medium additives,  Process Biochemistry, 2010, 45:880-886

32  Sheng Chen, lingqia Su, Susan Billig, Wolfgang Zimmermann, Jian Chen*, Jing Wu*, Biochemical characterization of the cutinases from Thermobifida fusca, Journal of Molecular Catalysis B: Enzymatic, 2010, 63(3-4):121-127

33  Ping Wang, Qiang Wang, Xuerong Fan*, Li Cui, Jiugang Yuan, Sheng Chen, Jing Wu, Effects of cutinase on the enzymatic shrink-resist finishing of wool fabrics,  Enzyme and Microbial Technology, 2009, 44(5):302-308

34  Sheng Chen, Xing Tong, Ronald W. Woodard, Guocheng Du, Jing Wu*, Jian Chen*, Identification and Characterization of Bacterial Cutinase, Journal of Biological Chemistry, 2008, 283(38):25854-25862

 

二、专利情况

1      吴敬,洪若宇,宿玲。玛. 一种利用角质酶处理造纸白水的方法. 中国, ZL 201611203776.9

2      吴敬, 陈晟, 杨玉路, 一种利用环糊精葡萄糖基转移酶生产偶合糖的方法, 中国, ZL201410677531.4

3       吴敬,宿玲。玛,熊艳军. 一种提高AA-2G转化率的环糊精葡萄糖基转移酶突变体.中国, ZL201410779194.X

4      吴敬,陈晟,段绪果,陈坚.一种普鲁兰酶突变体及其制备方法. 中国, ZL201310471021.7

5      吴敬,段绪果,陈晟,陈坚. 一种重组淀粉脱支酶发酵生产工艺.中国, ZL201210519218.9

6      吴敬,段绪果,吴丹,陈晟,陈坚. 一种耐热酸性普鲁兰酶生产菌株及其筛选方法.中国, ZL201310079335.2

7      吴敬,李祝,吴丹,陈晟,陈坚. 一种α-淀粉酶高产菌株及其发酵生产淀粉酶的方法.中国, ZL201310076922.6

8      吴敬,宿玲。玛,陈坚. 一种基质表达T. fusca角质酶的方法. 中国, ZL201210241426.7

9      吴敬,陈晟,段绪果,陈坚. 一种通过促进活性蛋白聚集体解聚提高淀粉脱支酶产量的方法.中国, ZL201210035298.0

10   吴敬,陈晟,李祝,陈坚. 一种耐热α-淀粉酶及其基因工程菌的构建方法. 中国, ZL201210274173.3

11   吴敬,陈晟,何洁,宿玲。录. 一种宽pH作用范围木聚糖酶及其应用.中国, ZL201210284346.X

12    吴敬,张帆,邓辉,陈晟, 陈坚. 一种葡萄糖异构酶的固定化方法.中国, ZL201210581801.2

13    吴敬,陈晟,段绪果,陈坚. 一种酸性耐热异淀粉酶基因工程菌及其应用.中国, ZL201110459137.X

14    吴敬,宿玲。玛,陈坚. 一种将胞内蛋白基质表达的方法和其应用.中国,ZL201210045965.3

15    吴敬,陈晟,段绪果,陈坚. 一种普鲁兰酶突变体及其制备方法.中国,ZL201210256804.9

16    吴敬,陈晟,邓辉,陈坚. 一种葡萄糖异构酶突变体及其应用.中国,ZL201110406411.7

17    吴敬,陈晟,吴玉飞,陈坚,夏泽华. 一种β-半乳糖苷酶的突变体及其制备方法和应用.中国,ZL201110247338.3

18    吴敬,陈晟,张瑶,陈坚,夏泽华. 一种耐热角质酶-CBD融合酶及其突变体和应用.中国,ZL201110295384.0

19    吴敬,陈晟,王磊,陈坚,夏泽华. 一种化学修饰的生麦芽糖淀粉酶.中国,ZL201110313483.7

20    吴敬,陈晟,陈坚. 一种角质酶的突变体及其制备方法.中国,ZL201110139321.6

21    吴敬,陈晟,吴丹,赵志军,陈坚,一种大肠杆菌胞内表达载体及应用,中国, ZL201010226274.4

22   吴敬,陈晟,宿玲。录,吴丹. 一种高效分泌表达重组角质酶的基因工程菌及其构建方法.中国,ZL200910260984.6  

23   吴敬,吴丹,郑贤良,陈坚,陈晟. 一种环糊精葡萄糖基转移酶复配酶制剂.中国,ZL200910260985.0    

24   吴敬,吴丹,王宁,陈坚,陈晟. 一种生物法生产α-环糊精的生产工艺.中国,ZL200910260986.5   

25   陈坚,吴敬,陈晟. 一种耐热角质酶及其编码基因和表达.中国,ZL200710026074.2

26    吴敬,陈坚,张瑶,陈晟. 一种耐热角质酶-CBD的制备及其在棉纤维精练中的应用.中国,张瑶ZL200910033667.0 

27   陈坚,吴敬,陈晟. 一种高温角质酶及其基因序列.中国,ZL200810020124.0 

 

 

三、主持科研项目

1.    大肠杆菌I型途径基质分泌重组工业酶的机理探究及效能强化,国家自然科学青年基金项目(No.31100048), 2012.1-2014.12

2.    联合酶法高效转化淀粉制备环糊精关键技术的研究.江苏省科技支撑项目(BE2011711). 2011-2014

3.    大肠杆菌II型途径基质分泌重组蛋白的效能提升, 高等学校博士学科点专向科研基金项目. (20110093120010). 2012-2014

4.    新型高效角质酶产业化制备关键技术,江苏省产学研前瞻性联合研究项目(BY2015019-18),2015-2017

5.    寡聚化介导的糖基转移酶热稳定性分子改造机制探究,国家自然科学基金面上项目(No.31571776), 2015.1-2019.12

6.    4,6/3-α-葡萄糖基转移酶转化淀粉制备抗性糊精的分子机制研究,(No. 31972032), 2020.1-2023.12

 

四、获奖情况

2011  教育部高等学校科学技术进步一等奖 教育部

环糊精葡萄糖基转移酶的制备和应用技术,排名第三

2018  中国商业联合会科技进步奖特等奖 中国商业联合会

新型高效淀粉脱支酶的创制及工业化应用,排名第三

2019 国家技术发明二等奖 国务院

“淀粉加工关键酶制剂的创制及工业化应用技术”,排名第三

 

 


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